MMP-3和TIMP-3在膀胱移行细胞癌中的表达及意义

Expression and significance of MMP-3 and TIMP-3 in transitional cell carcinoma of bladder

目的研究基质金属蛋白酶3（MMP-3）及其抑制物组织基质金属蛋白酶抑制剂3（TIMP-3）在膀胱移行细胞癌中的表达及其临床意义。方法通过免疫组化Elivision法检测48例膀胱移行细胞癌组织及20例正常膀胱组织中的MMP-3和TIMP-3的表达。结果MMP-3和TIMP-3在正常膀胱组织中不表达，随着肿瘤分级和分期的增高，MMP-3表达增高，但差异无统计学意义（P〉0．05）。TIMP-3表达降低，但在肿瘤分级中差异有统计学意义（P〈0．05）。结论MMP-3的高表达，TIMP-3的低表达与膀胱移行细胞癌的恶性程度有关，在膀胱移行细胞癌的发生发展中起重要作用，可作为临床判断膀胱移行细胞癌的恶性程度及预后的重要参考指标。

Objective To study the expressions and significances of matrix metalloproteinases-3 （MMP-3） and tissue-inhibitor of matrix metalloproteinase-3 （TIMP-3） in transitional cell carcinoma of bladder. Methods Im- munohistochemistry was used to detect the expressions of MMP-3 and TIMP-3 in 48 cases of transitional cell carci- noma of bladder and 20 normal bladder tissues. Results MMP-3 and TIMP-3 in normal bladder tissues did not ex- press. The more grading and staging, the more MMP-3 expression, but no statistical significance （P 〉 0.05）, by contrast, TIMP-3 expression decreased and has statistically significant in the tumor grading （P 〈 0.05）. Conclusions High expression of MMP-3 and low expression of TIMP-3 is related to the malignancy of transitional cell carcinoma of bladder and may play an important role in the development of transitional cell carcinoma of blad- der, as an important reference to judge the degree of malignancy of transitional cell carcinoma of bladder and prognostic indicator.