徐闻牡丹珊瑚共附生真菌分离鉴定及多样性分析

Isolation and Identification of Endophytic Fungi Associated with Pavona Lamarck of Xuwen Natural Reserve

本文以牡丹珊瑚为原料,选用2种培养基,采用平板涂布法培养分离菌株,对照真菌鉴定手册,根据菌株的菌落大小、颜色、生长速率等菌落形态以及菌丝体和孢子的个体形态进行分离鉴定,并根据ITS-rRNA基因序列特征进行分子鉴定,依照测序结果建立系统发育树并进行种群多样性分析。结果显示从该样品中共分离到10株共附生真菌,分属于5个属,其中曲霉属有3株、肉座菌属有3株、有2株属于青霉属、1株顶枝孢属及1株白腐菌属。说明曲霉属和肉座菌属是牡丹珊瑚上的优势菌属。牡丹珊瑚与其它种类珊瑚比较分离到的真菌数量较少,但均匀度比较高。从牡丹珊瑚上分离到的肉座菌属和白腐菌属在海洋真菌的研究中未见报道。

To study the diversity of fungi derived from Pavona lamarck, spread-plate method and two different media were used to cultivate and isolate the strains. The strains was identified from the colony morphology, the individual form of mycelium and spores referring to fungi identification manual. Molecule was identified according to the ITS-rRNA gene sequence feazes. Based on the result of sequencing, a phylogenetic tree was build for population diversity analysis. 10 symbiotic fungis were isolated from the Pavona lamarck, belonging to 5 generas including 3 Aspergillus spp., 3 Hypocrea spp., 2 Penicillium spp., 1 Cladosporium spp. and 1 Phlebia spp., indicating that Aspergillus and Hypocrea were the dominant fungi. Compared to other corals, the fungis isolated from Pavona lamarck were fewer, but had a high degree of urtiformity. There was no report on marine fungis.