丹参多酚酸盐对大鼠骨髓间充质干细胞增殖及血管内皮生长因子表达的影响

Effect of salvianolate on cell proliferation and levels of VEGF of rat bone marrow mesenchymal stem cells

目的观察中药丹参多酚酸盐对SD大鼠骨髓间充质干细胞的增殖及血管内皮生长因子(VEGF)表达的影响,以探讨其对促骨折愈合的可能机制。方法将0.5 mg/ml浓度丹参多酚酸盐与大鼠骨髓间充质干细胞共培养,对照组为无药物单位培养的大鼠骨髓间充质干细胞。培养48小时镜下观察细胞情况,1、3、5、7天行MTT检测细胞增殖情况。行实时荧光定量PCR检测方法,对培养7天的两组细胞的VEGF mRNA水平进行检测分析。结果共培养48小时后骨髓间充质干细胞生长密集程度明显优于对照组;MTT检测也显示实验组细胞增殖情况优于对照组;实验组VEGF mRNA表达高于对照组。结论丹参多酚酸盐能促大鼠骨髓间充质干细胞增殖,并提高其VEGF的表达。可能是其促骨折愈合的作用机制之一。

Objective To investigate the possible fracture healing mechanism of salvianolate through improving of proliferation and vascular endothelial growth factor（VEGF） levels of rat bone marrow mesenchymal stem cells（BMSC）. Method This experiment adopted the method of cell culture in vitro. Salvianolate（0. 5mg/ml） was co-clcutured with rat BMSCs, BMSCs was cultured without Salvianolate as the control. The growth of the cells was observed under the microscope after 48 hours after culture. 1, 3, 5, 7 days later, MTr was used to detect the cell proliferation. VEGF mRNA levels of BMSCs were detected and analysed by quantitative real time polymerase chain reaction （qRT-PCR） at 7 days after cell culture.Results The cell growth intensity in the co-cuhure group was significantly better than in the control group observed under microscope after 48 hours after culture. MTI＂ assay showed that cell proliferation in experi- mental group were higher than in the control group after 3 days of cell culture. VEGF mRNA level in the co-culture group were significantly better than the control group after 7 days of cell culture. Conclusion Salvianolate can promote rat bone marrow mesenchymal stem cell proliferation, and increase the expression of VEGF. This might be one of the possible mechanisms of promoting fracture healing.