LAS对雄性小鼠生殖功能毒性作用的研究

Study on the Toxicity of LAS to Male Mice Reproductive Function

目的探讨直链烷基苯磺酸钠(LAS)对雄性小鼠生殖功能的毒性作用。方法昆明种雄性小鼠给予LAS(630mg/kg,315mg/kg)灌胃,每日1次,连续经口染毒,同时设对照组。在染毒后4周、8周进行精子质量分析,光镜观察睾丸组织的形态学变化,透射电镜观察睾丸超微结构变化。结果实验组与对照组相比,染毒4周后精子密度与活力下降,畸形率上升,畸形主要出现在颈部,精子胞浆小滴增多;染毒8周精子密度及活力下降及畸形率升高更显著,异常改变均有浓度依赖性。组织学观察发现实验组小鼠染毒4周时生精小管内各级生精细胞减少,生精细胞排列紊乱,随着染毒时间延长及染毒浓度的增加其异常改变更显著;超微结构观察显示睾丸组织支持细胞、间质细胞、初级精母细胞内线粒体广泛空泡样改变,亦可见明显的时间与浓度依赖关系。结论亚慢性LAS染毒对雄性小鼠生殖功能具有明显的毒性损伤作用,且具有时间与浓度依赖性。

Objective To investigate the toxicity effect of LAS on male mice reproductive function .Methods Kun-ming male mice were treated P.O.with LAS 630mg/kg,315mg/kg（1/2,1/4 LD50）,one time per day.The control mice were treated with normal sodi-um.Sperm quality analysis,light microscopy and transmission electron microscopy of the testis were assayed at four and eight weeks .Results Compared to the control group,the concentration and vitality of sperm in treated groups significantly reduced and deformed sperm numbers increased when male mice were treated after four weeks ,while more affected after eight weeks .Compared with the control group ,the higher of the concentration and vitality of sperm was significant .The percentage of sperm cytoplasmic droplets increased .The changes of spermary tissue under microscope and transmission electron microscope revealed the damage of LAS to the testis .Conclusion The study showed that LAS af-fected spermary cell significantly.