奥卡西平对癫痫幼鼠海马神经元神经细胞黏附分子表达的影响

Effects of Oxcarbazapin on the Expression of Nerve Cell Adhesion Molecule in the Hippocampus of Immature Rats with Epilepsy

目的观察奥卡西平(OXC)对慢性癫痫幼鼠海马神经元中神经细胞黏附分子(NCAM)表达的影响。方法将50只21日龄SD大鼠随机分为5组:生理盐水(NS)组、戊四氮(PTZ)组、OXC低剂量(100mg/kg,LDOXC)组、OXC中剂量(200mg/kg,MDOXC)组、OXC高剂量(300mg/kg,HDOXC)。建立不同剂量的OXC干预癫痫幼鼠模型。连续用药21d,观察幼鼠体质量、行为学表现;免疫组织化学法观察NCAM阳性细胞的表达;Real-time PCR检测海马组织中NCAM mRNA的表达。结果 LDOXC、MDOXC、HDOXC组癫痫发作时间延长,发作程度减低(P<0.01);LDOXC、MDOXC、HDOXC组海马组织中NCAM阳性细胞、NCAM mRNA的表达降低(P<0.05)。结论 OXC可抑制癫痫幼鼠海马神经元中NCAM的表达,并存在一定的剂量依赖性。OXC可能通过减少NCAM的表达,抑制PTZ致幼痫鼠海马神经元发生,减轻癫痫引起的脑损伤。

Objective To explore the effects of oxcarbazapine(OXC)on the expression of NCAM in hippocampus of immature rats with epilepsy . Methods A total of 50 male SD rats around 19 to 21 days of age were randomly allocated into 5 groups :normal saline (NS)group ,pentylenetetrazole (PTZ) group ,low-dose OXC group(100 mg/kg ,LDOXC) ,middle-dose OXC group(200 mg/kg ,MDOXC) and high-dose OXC group(300 mg/kg ,HDOXC) . The animal model of rats with epilepsy treated by different doses of OXC was established . After twenty-one days administration ,changes in behavioral performance were recorded ,and the NCAM protein level and mRNA in hippocampus were examined by immunohisto-chemisty and Real-time PCR respectively . Results Compared with PTZ group ,latent time and degree of epileptic seizures induced by PTZ were prolonged and alleviated in OXC groups (P<0 .01) ,the expression of NCAM in the astrocyte and NCAM mRNA in hippocampus were depressed in OXC groups (P<0 .05) . Conclusion NCAM may correlate with epilepsy closely .OXC could decrease the expression of NCAM with a dosage dependent manner .OXC may alleviate the brain damage caused by epilepsy .