经肝内注射肿瘤细胞建立小鼠肝癌实验模型的效果观察

Observation of the effect of tumor model establishment for hepatocellular carcinoma in mice via tumor cell intrahepotic injection

目的探讨经肝内直接注射肝癌细胞建立小鼠肝癌原位模型的效果。方法选取Balb/c小鼠来源的ML-3肝癌细胞经肝内直接注射接种至肝脏实质内,与经脾注射肝癌细胞相比较,对其肝内成瘤情况进行观察。结果肿瘤细胞接种第6天起,经脾脏注射组以及经肝脏注射组小鼠均不同程度出现体重减轻、腹水、黄疸以及腹腔可触及包块。在经脾脏注射组,15只小鼠中仅有3只长出肝脏肿块,其余均腹腔种植转移。在经肝脏直接注射组,15只小鼠中有10只长出肝癌,其中伴腹腔种植转移1只。结论经肝脏直接注射肿瘤细胞较经脾脏注射法能更有效诱导建立肝癌原位模型。

Objective To establish an orthotropic tumor model for hepatocellular carcinoma in mice via tumorcell intrahepotic injection and evaluate the effect. Methods ML-3 hepatocellular carcinoma cells derived from Balb/c mice were injected into the mice through intrahepotic injection and intra-spleen injection. The tumor formation and the survival of the mice were observed. Results The weight reduction, ascites, jaundice and abdominal mass were found on day 6 of tumor injection. Liver tumor was oberserved in 3 mouse by introspleen injection, and implantation metastasis were found in the other 12 mice; while among the 15 mice through intrahepotic injection, liver tumor were found in 10 mice and among them 9 mice were without implantation metastasis. Conclusion The orthotropic tumor model for hepatocellular carcinoma in mice is successfully established by intrahepotic injection rather than intra-spleen injection.