常用补虚药水煎液对小鼠抗应激作用的比较研究

Comparative study on mice anti stress effect with common tonic medicine decoction

目的通过低温游泳、常压缺氧的实验方法,分别比较人参、党参、黄芪及枸杞子等常见补虚药水煎液对小鼠抗应激反应能力的影响。方法将50只小鼠随机分为人参水煎液组、党参水煎液组、黄芪水煎液组、枸杞子水煎液组和生理盐水组,每组10只,按照0.4mL/10g灌胃1h后,进行低温游泳及耐缺氧实验。结果人参、党参、黄芪及枸杞子等常见补虚药水煎液均能显著增强小鼠抗应激反应的能力;人参提高小鼠抗应激反应的作用显著优于黄芪和枸杞子,而在低温游泳实验中人参和党参的作用差异无统计学意义。结论补虚中药能显著提高小鼠抗应激反应,但不同补虚中药的作用是有显著差异的,临床使用补虚中药时,需要考虑补虚中药的合理运用。

Objective Comparative study on mouse anti stress effect with common tonic medicine decoction of ginseng,root of pilose asiabell,root of membranous milkvetch,ripe fruit of barbary wolPoerry by the low temperature swim test and hypoxia tolerance test. Methods 50 mice were randomly divided into 5 groups： ginseng decoction group,root of pilose asiabell decoction group,root of membranous milkvetch decoction group,ripe fruit of barbary wolfberry decoction group and physiological saline group,lO mouse in each group. Mice in treatment were lavaged with decoction and physiological saline （0.4mL/10g）. After 1 hours mice were swimmed in low temperature and hypoxia tolerance test. Results Mice anti stress ability were significantly enhance by ig decoction of ginseng,dangshen,astragalus root and wolfberry fruit. Mice anti stress reaction were treatment by decoction of ginseng was better than that of astragalus root and wolfberry fruit.There was no significant difference between the ginseng and root of pilose asiabell in low temperature swimming test. Conclusion Mice anti stress response can significantly improved by treatment tonic chinese medicine,but different effect on mice with tonic Chinese traditional medicine were significant differences. Difference effect were considered when tonic chinese medicine were used in the clinical.