HPLC法测定复方鲜竹沥液中水杨酸的含量

Determination of salicylic acid in compound fresh bamboo juice by HPLC

目的建立复方鲜竹沥液中水杨酸的测定方法。方法以X—Bridge C18柱（4．6×250mm，5μm）为色谱柱；乙腈一0．5％三乙胺与0．5％磷酸的混合溶液（20：80）为流动相，检测波长：210nm；流速：1．0mL／min。结果水杨酸在0．666～266．4μg／mL范围内呈良好的线性关系（r=0．9999），平均回收率为97．0％，RSD为0．8％（n=6）。结论本方法简便可行，重现性好，可用于复方鲜竹沥液的质量控制。

Objective To establish a method for determination of salicylic acid in compound fresh bamboo juice. Methods The chromatographic conditions included a X-Bridge C18 column（4.6×250mm,5μm）, the mobile phase of acetonitrile, 0.5% triethylamine and 0.5% phosphoric acid （20 ： 80）.The detection wavelength was 210nm and the flow rate was 1.0mL/min. Results There was a good linear relationship between salicylic acid in the range of 0.666-266.4μg/mL（r=0.9999）, the average recovery was 97.0% and RSD=0.8% （n=6）. Conclusion The method is simple and feasible, with good reproducibility, and can be used for quality control of compound fresh bamboo juice.