摘要
采用琼脂打孔法和微量二倍稀释法比较了桑黄液体发酵菌丝体的不同溶剂提取物及不同极性部位对5种常见致病菌的抑菌作用,并初步探讨了热处理、紫外线和介质pH值对其抑菌作用的影响。结果表明:桑黄菌丝体5种提取物均对测试菌株具有较好的抑菌活性,其中甲醇提取物抑菌活性最佳。在甲醇提取物各极性部位中,乙酸乙酯萃取部的抑菌活性最强。热处理及紫外线照射对提取物抑菌活性没有明显影响;随着介质pH值的增大,提取物抑菌活性呈下降趋势。
Antibacterial activity of different extracts from fermentation mycelia of Phellinus igniarius （L.ex Fr.） Quel against 5 common pathogenic bacteria were tested by agar diffusion and double dilution methods. Meanwhile, effects of thermal treatment, ultraviolet and pH on antibacterial activity of the extracts were also investigated. The results indicated that methanol extract had the best antibacterial activity in the 5 extracts. And the ethyl acetate fraction reached the best activity in the fractions from methanol extract. The antibacterial activity of extracts was stable to temperature and ultraviolet, and decreased with pH value increasing.
出处
《广东农业科学》
CAS
CSCD
北大核心
2013年第18期69-72,共4页
Guangdong Agricultural Sciences
基金
广东省自然科学基金博士启动项目(粤科基字[2010]3号)
广东省农科院院长基金(201006)
关键词
桑黄
菌丝体
发酵
提取物
抑菌活性
Phellinus igniarius
mycelia
fermentation
extract
antibacterial activity
