非小细胞肺癌组织中PTEN、NF-κB的表达及临床意义

The expression and clinical significance of PTEN and NF-κB in NSCLC tissues

目的检测非小细胞肺癌(NSCLC)组织中张力蛋白同源的磷酸酶基因(PTEN)和核因子κB(NF-κB)的表达及其与临床病理特征及预后的关系。方法运用免疫组织化学法检测PTEN、NF-κB在肺癌组织以及癌旁正常肺组织中的表达情况。结果 PTEN蛋白在肺癌组织中的阳性表达率明显低于癌旁正常肺组织(P<0.05);而NF-κB蛋白在肺癌中的阳性表达率,明显高于癌旁正常肺组织(P<0.05)。PTEN、NF-κB的阳性表达率与临床病理分期、组织分化程度及术后生存期有关(P<0.05),而与性别、年龄、吸烟史、肿瘤大小及病理类型无关(P>0.05);临床病理分期低、组织分化好的患者生存时间较长,差异有统计学意义(P<0.05)。此外,肺癌中PTEN与NF-κB呈负相关(rs=-11.30,P<0.05)。结论PTEN基因的失活、NF-κB基因的活化可能促进了肺癌的发生、发展和侵袭转移的进程,可作为临床评价肿瘤恶性程度及预后的指标。

Objective To detect the expressions of phosphatase and tensin homology deleted on chromosome ten （ PTEN ） and nuclear factor-KB （NF-KB） in non-small-cell lung carcinoma （NSCLC） tissues, and to explore their correlation with clinical pathological characteristics as well as patient＇ s prognosis. Methods The immunochemistry method was used to detect the expressions of PTEN and NF-KB in NSCLC tissues and adjacent nontumorous tissues. Results The positive rates of PTEN expression in lung cancer tissues were 24.42% （21/ 86） ,which were significantly lower than 72.09% （62/86） in adjacent nontumorous tissues （ P 〈 0.05 ）, however,the positive rates of NF-KB expression in lung cancer tissues were 63. 95% （55/83）, which were significantly higher than 25.58% （22/86） in paracancerous tissues （ P 〈 0.05 ）. The positive rates of PTEN and NF-KB expressions were related to clinical pathology staging, differentiation degree of tissue and survival periods after operation, however,which were not correlated with gender, age, smoking, tumor size and histological grading （ P 〉 0. 05 ） , however , the expression of PTEN was negatively correlated to that of NF-KB （ rs = -11.30, P 〈 0.05）. Conclusion The inactivation of PTEN gene and activation of NF-KB gene may promote the pathogenesis, development, invasion and metastasis of lung cancer, which can be used as the indexes to evaluate tumor＇ s malignant degree and prognosis.