大麻二酚抗类风湿关节炎作用研究

Studies on anti-rheumatoid arthritis effect of cannabidiol

目的观察工业大麻中提取分离得到的大麻二酚的抗类风湿关节炎作用。方法建立角叉菜胶致大鼠急性炎症模型和佐剂诱导的大鼠关节炎模型,测定大麻二酚5、7.5、10和20 mg/kg各剂量组大鼠灌胃后不同时间的肿胀值变化,并与艾瑞昔布阳性对照组比较。结果与角叉菜胶致大鼠急性炎症模型组相比,大麻二酚各剂景给约组大鼠肿胀值均下降,并随给药剂量和时间的增加抗炎作用逐渐增强,其小20 mg/kg大麻二酚给药后3 h肿胀值和肿胀率均较阳性对照组降低,但差异均无统计学意义;与佐剂诱导的大鼠关节炎模型组相比,大麻二酚各给药组显示出一定的量效关系,且10 mg/kg大麻二酚剂量组给药后第12 d以及20 mg/kg剂量组给药后第10和12 d的肿胀值和肿胀率均显著降低(P<0.05,P<0.01),而大麻二酚各剂量组与阳性对照组相比差异并无统计学意义。结论大麻二酚对角叉菜胶致大鼠急性炎症和佐剂诱导的大鼠关节炎模型均有一定作用。

Objective To observe the anti-inflammatory effect of cannabidiol isolated from Cannabis sativa L. Methods An acute inflammation model induced by carrageenan and an arthritis model induced by Freund＇s complete adjuvant in rats were established. The swelling values of rats in different time administrated with cannabidiol 5, 7.5, 10, 20 mg/kg including imrecoxib as positive control, were measured. Results In carrageenan induced acute inflammation model group, the swelling values of rats in each cannabidiol group were reduced, and with increasing dose and time, cannabidiol showed a marked dose-dependent and time-dependent anti-inflammatory effect. Especially, the swelling value and rate in 20 mg/kg group at 3 h after administration of cannabidiol was decreased than that in imrecoxib positive group, but there was no significant difference between two groups. In adjuvant-induced arthritis model group, the swelling value and rate on administration cannabidiol at dose of 10 mg/kg （P（0.05） for 12 d were decreased significantly, and there were both decreased significantly for dosing 20 mg/kg ofcannabidiol after 10 d and 12 d respectively （P〈0.05, P〈0.01）. In addition, all cannabidiol groups showed quantitative activity relationship, but there was no statistical difference between cannabidiol groups and imrecoxib positive control group. Conclusion Cannabidiol has anti-inflammatory effect on tests induced by carrageenan and Freund＇s complete adjuvant.