男性因素在辅助生殖技术中对反复种植失败的影响

The effect of male factors on repeated implantation failure in assisted reproductive technology

目的探讨体外受精-胚胎移植(IVF-ET)技术中反复种植失败(RIF)的男性影响因素,分析RIF组与正常对照组的临床资料、DNA碎片化指数(DFI)、精液常规参数、辅助助孕指标的差异。方法选取接受IVF-ET技术助孕患者共49例,其中RIF 19例作为病例组;临床妊娠30例作为对照组,采用Annexin V-FITC/PI精子双染,通过流式细胞仪器(FCM)检测精子亚群所占的比例。结果 2组男方年龄、体质量指数(BMI)、吸烟史、饮酒史、精液量、密度、前向运动比率(PR)、形态及DFI差异无统计学意义(P>0.05);2组女性年龄、获卵数、受精率、卵裂率、可移植胚胎数、优质胚胎数及移植日子宫内膜厚度(Em)差异无统计学意义(P>0.05)。结论影响RIF发病的男性因素较多,DFI、BMI及生活方式可能不是RIF发生的独立因素。

Objective To explore the male factors on repeated implantation failure（RIF） ,and analyze the differences of sperm DNA fragmentation index （DFI）, sperm parameter and IVF -ET outcome during two groups. Methods The object who accepted in - vitro fertilization -embryo transfer （IVF- ET） were divided into two groups （n = 49 ） ：the group of successful clinic pregnancy （n = 30 ） and RIF group （n = 19 ）. DNA fragmentation was stained by Annexin V -FITC/PI assay, the results were analyze by flow cytometry （FCM） to determine the ratio of subgroup. Results No significant differences were found between male age, smoking history, BMI, drinking history, sperm volume, parameters and DFI （ P 〉 0.05 ）. There was no relationship between female age, number of picked - up o-ocyte, fertilization rate, cleavage rate, available embryos, high quality embryos in two groups （ P 〉 0.05 ）. Conclusion Many male factors lead to the development of RIF, age, DFI, BMI and living habit may not be independent factor.