稠油厌氧降解产甲烷菌群的组成及产甲烷特性

Composition and Methanogenic Characteristics of Microbial Consortium for Heavy-Oil Anaerobic Degradation

从胜利油田聚驱稠油油藏采出水中富集得到一组55℃下生长的稠油厌氧降解产甲烷混合菌群SL-7,利用变性梯度凝胶电泳(DGGE)技术分析混合菌群的组成,并对其产甲烷特性进行了分析。结果表明:混合菌群SL-7由6株主要的单菌组成,其中2株单菌可分离培养,分别来自Firmiucutes和β-Proteobacteria,另外4株单菌为不可分离培养的菌株。经过270d培养,混合菌群SL-7所产有机气体中,甲烷产生量达到最大值,为1110μmol,占95.2%,其它气体(异丁烷、正戊烷、2,2-二甲基丁烷、2-甲基戊烷等)占4.8%,此时混合菌群SL-7对稠油的降解率达到30.6%。高效稠油降解产甲烷混合菌群的获得为残余低品位稠油微生物气化奠定了基础。

The microbial consortium SL-7 which is enriched from produced water of polymer flooding of heavy-oil reservoir in Shengli Oilfield cultured at 55 ℃,its composition is analyzed by using denaturing gradient gel eleetrophoresis （DGGE）,and then the methanogenic characteristics are analyzed. The results show that. the microbial consortium SL-7 contains 6 major components,including two single strains can be isolated and cul- tured,respectively from Firmiucutes and β-Proteobacteria, four other single strains can not be isolated. After 270 d culture,the microbial consortium SL-7 has produced organic gases and methane which is accounting for 95.2% reaches a maximum value of 1110 μmol, other gases （isobutane, n-pentane, 2,2-dimethyl butane, 2- methylpentane etc. ） achieves 4.8%. The degradation rate of heavy-oil by microbial consortium SL-7 reaches 30.6 %. The high efficient microbial consortium can lay the foundation for the residual low-grade heavy-oil gasification.