金纳米粒子对聚合酶链式反应体系中长链DNA特异性扩增的效应

EFFECT OF AU NANOPARTICLES ON THE SPECIFIC AMPLIFICATION OF LONG DNA FRAGMENTS IN POLYMERASE CHAIN REACTION

以乙烯受体基因ETR1（2500bp）和山梨醇脱氢酶基因（SDH）（1100bp）为材料，研究了不同浓度金纳米粒子对聚合酶链式反应（PCR）体系中大于1000bp的长链DNA特异性扩增的效应。结果表明，金纳米粒子显著增强了ETR1和SDH在PCR时的特异性扩增，有效降低了非特异性扩增。在ETR1和SDH的PCR中，金纳米粒子适宜浓度为0．4nmol·L^-1。在50℃-62℃的退火温度范围内，金纳米粒子均能够显著增强ETR1和SDH的特异性扩增。因此，金纳米粒子不仅提高了1000bp以上的长链DNA在PCR扩增的特异性，而且拓宽了PCR的退火温度范围。

Effects of different concentrations of Au nanoparticles on the specific amplification of long DNA fragments （more than 1000 bp） in polymerase chain reaction （PCR） were studied, using ethylene receptor ETR1 gene （2500 bp） and sorbitol dehydrogenase （SDH） gene （1100 bp） as materials. The results suggested that Au nanoparticles could significantly enhance the specific amplification of ETRI and SDH, and effectively reduce the nonspecific amplification in PCR. The proper concentration of Au nanoparticles was 0.4 nmol · L^-1 for ETR1 and SDH in PCR. When the annealing temperature was varied from 50 to 62 ℃, the specific amplifications of ETR1 and SDH in PCR could be increased as usual by Au nanoparticles. Therefore, Au nanoparticles could not only improve the specific amplification of long DNA with more than 1000 bp, but also broaden the range of an- nealing temperature in PCR.