利用SSR标记分析烤烟轮回选择群体的遗传多样性

Genetic Diversity Analysis of Recurrent Selection of Flue-cured Tobacco Populations by SSR Marker

以烤烟轮回选择3个世代群体为材料,利用SSR标记技术分析不同世代材料的遗传多样性。利用16个原始材料从350对SSR引物中筛选出条带清晰、多态性稳定的27对引物并在C0、C1、C2 3个群体各选取46个单株进行分析。结果显示,改良群体比基础群体的多态性位点数和基因杂合度略低,但差异不明显;C1和C2群体的平均遗传距离与C0相比有所减小,但变化不明显,同时经过重组交换,也增加了部分个体间的异质性。整体上来看,轮回选择的改良群体与基础群体的遗传变异相似,通过两轮选择,改良群体能较好的保持原始群体的遗传多样性。

Using SSR makers, this study analyzed the genetic diversity of three populations from recurrent selection of flue-cured tobacco for material. 350 pairs of SSR primers were screened by 16 original lines, and 27 pairs of primers showed clear bands and stable polymorphism. PCR analyses were carried out among three populations of CO, C1 ,C2,46 individuals from each population were selected for analysis. The results showed that the polymorphic loci and heterozygosity of the improved groups were slightly lower than those of the origi- nal group, but the difference was not significant. The average genetic distance in C1 and C2 groups was smaller than in CO group,but it did not change significantly. The genetic distance of some individuals from the im- proved group was enlarged by recombination. The results indicated that the improved group by recurrent selec- tion had similar genetic variation to the original group, and the improved group could maintain approximate ge- netic diversity of the original group.