摘要
目的 :研究血尿胶囊中原儿茶酸的定量分析方法。方法 :样品用乙醇 醋酸 (10∶1)回流提取 ,无水乙醇溶解点于硅胶H薄层板上。以氯仿 丙酮 甲醇 醋酸 (7∶2∶1.5∶0 .5 )为展开剂 ,用薄层色谱扫描仪λs=5 30nm下扫描。结果 :平均回收率 10 1.4%。结论 :本方法灵敏、准确、专属性好 ,可做为该制剂的含量测定及质量控制。
Objective: To establish a quantitative method for determination of protocatechuic acid in Xueniao Capsules. Methods: The capsules were extracted with alcohol acetic acid (10∶1) and then solved with absolute alcohol. The sample was applied on a silica H plate, developed and separated in the solution of chloroform acetone methanol acetic acid (7∶2∶1.5∶0.5). The separated spots were determined by TLC scanning at 530nm(λ s). Results: The average recovery was 101.4%. Conclusion: This method for determination of protocatechuic acid in Xueniao Capsules was sensitive, accurate and specific. It could be used as quality control for Xueniao Capsules.
出处
《中成药》
CAS
CSCD
2000年第12期839-840,共2页
Chinese Traditional Patent Medicine
