全骨髓贴壁培养兔骨髓间充质干细胞的初步研究

A Preliminary Study on the Way of the Whole Bone Marrow Adherent Culturing Rabbit Bone Marrow Mesenchymal Stem Cells

目的:探讨兔骨髓间充质干细胞作为组织工程种子细胞修复骨缺损的可能性。方法:选取2月龄约2.5 kg重健康新西兰兔8只,采用全骨髓贴壁培养法体外分离、培养兔骨髓间充质干细胞,通过倒置相差显微镜观察、MTT检测、细胞贴壁率检测观察细胞生物学特性。结果:全骨髓贴壁培养法培养出的原代骨髓间充质干细胞活力良好,细胞数量经传代后扩增,且细胞纯度提高,但细胞扩增速度较慢。结论:全骨髓贴壁培养法简便易行,能有效的在体外获得原代骨髓间充质干细胞。所提取的细胞活性良好、纯度较高,能用作组织工程种子细胞治疗骨缺损。

Objective： To explore the feasibility of using rabbit bone marrow mesenchymal stem cells （BMSCs） as seed cells to repair bone defect in tissue engineering. Methods： Selects eight 2 - month - old of 2. 5kg each New Zealand rabbits, BMSCs are isolated from bone marrow blood and purified by the way of the whole bone marrow adherent culture and in vitro culture. The proliferation and growth characteristics were observed by the inverted microscope, MTT assay and cell adherent rate test. Results： Bone marrow mesenchy- mal stem cells showed good viability in vitro in primary and passage cultures, but slower proliferation rate. Conclusion： It is a simple and effective way to cultivate the BMSCs using whole bone marrow adherent culture method. The BMSCs can be used as the seed cells for meniscus tissue engineering.