三唑磷短期暴露对翡翠贻贝的毒性效应

Toxic effects of short-term triazophos exposure on mussel Perna viridis

采用半静态毒性试验方法研究了三唑磷(triazophos,OP)短期暴露下对翡翠贻贝(Perna viridis)的毒性效应。结果显示,在OP胁迫下,翡翠贻贝内脏团和外套膜超氧化物歧化酶(SOD)活性在胁迫前期均被抑制,随时间延长活性被诱导。低质量浓度OP(0.4 mg·L-1和2 mg·L-1)使翡翠贻贝内脏团和外套膜谷胱甘肽-S转移酶(GST)活性先降低后升高;高质量浓度(10 mg·L-1)GST活性降低。OP能诱导还原型谷胱甘肽(GSH)的产生,而高质量浓度(10 mg·L-1)也能抑制GSH产生。受OP胁迫影响,翡翠贻贝内脏团和外套膜丙二醛(MDA)均升高。内脏团总抗氧化能力(T-AOC)显著降低;而外套膜T-AOC会被OP所诱导。转入清水恢复72 h后仅外套膜MDA有所恢复。结果表明OP短期暴露会对翡翠贻贝抗氧化系统造成损伤。

A semi-static toxicity test was carried out to study the toxic effects of short-term triazophos （OP） exposure on mussel Perna viridis. The results showed that SOD activity of visceral mass and mantle were inhibited in early exposure stage, and then induced with exposure time progressed. Lower concentrations of OP （0.4 mg·L^-1 and 2.0 mg·L^-1 ） decreased GST activity in visceral mass and mantle, and then increased as the time went by; GST activity was reduced with higher concentration of triazophos （ 10. 0 mg·L^-1 ）. OP induced the production of GSH; while with the high concentration （ 10. 0 mg·L^-1）, it also inhibited GSH producing. Under OP exposure effect, MDA levels significantly increased in visceral mass and mantle. T-AOC was significantly reduced in visceral mass, while T-AOC was induced in mantle. OH radical capacity was reduced by OP, while T-AOC in mantle was induced as OP stress in- creased. After 72 h recovery in natural seawater, only MDA content in mantle restored. This study shows that the antioxidant defense system of P. viridis can be damaged by short-term triazophos exposure.