同时分析酒石酸美托洛尔和富马酸比索洛尔与阿替洛尔的气相色谱-质谱联用法研究

Simultaneous determinations of metoprolol tartrate and bisoprolol fumarate and atenolol by gas chromatography-mass spectrography

目的建立同时分析酒石酸美托洛尔、富马酸比索洛尔和阿替洛尔的气相色谱-质谱联用法。方法样品经N，O-双（三甲基硅烷）三氟乙酰胺衍生化后，用气相色谱-质谱联用仪进行分离与分析。结果3种心脏病常用药物酒石酸美托洛尔、富马酸比索洛尔和阿替洛尔的检出限分别为16．73、33．58、21．46mg／L，对应的线性范围均为0．05—1．00g／L，回收率为91．2％、98．8％、88．4％。结论建立了同时分离分析3种心脏病药物的方法，该方法样品处理简便，色谱分离完全，结果准确可靠，为后期心脏病患者尿液中的药物代谢组学研究提供了基础。

Objective To establish gas chromatography-mass spectrometry （GC/MS） method for the simultaneous determination of 3 drugs for heart disease including metoprolol tartrate, bisoprolol furnarate and atenolol. Methods The urine extracts were derivatized with N-methyl-N-（trimethylsilyl） trifluoroaeetamide at first, and then analyzed by GC-MS. Results The detection limits were 16.73 mg/L for metoprolol, 33.58 mg/L for bisoprolol and 21.46 mg/L for atenolol, respeetively. The linear ranges of metoprolol, bisoprolol and atenolol were in the range of 0.05-1.00 g/L, respectively. The recoveries of 3 heart disease drugs were in the range of 91.2% , 98.8% , 88.4%. Conclusion The GC/MS is applicable for the study of heart disease pharmaeo metabonomies.