化痰行瘀汤对肝纤维化大鼠组织病理学及羟脯氨酸含量的影响

Efficacy of Huatan Xingyu Decoction in treatment of hepatic fibrosis in rats

目的观察化痰行瘀汤对肝纤维化的疗效,并进一步探讨其抗纤维化的作用机制。方法取清洁级健康成年Wjstar大鼠60只,随机抽取10只作为正常对照组,其余50只采用经典模型复制后随机数字表法分为5组(造模过程中死亡11只),模型对照组(10只)、复方鳖甲软肝片组(7只)、和化痰行瘀汤低(7只)、中(7只)、高(6只)剂量组,分别以相应药物连续干预6周。采用样本碱水解法检测各组大鼠部分肝组织羟脯氨酸(Hyp)含量;并采用常规HE染色和苦味酸-天狼红染色观察肝纤维化程度。结果复方鳖甲软肝片组、化痰行瘀汤各剂量组能明显改善肝纤维化程度;化痰行瘀汤中剂量组的病理改变明显,明显降低肝组织Hyp含量(P<0.05)。结论化痰行瘀汤能降低肝组织Hyp含量,明显减轻病理改变,有效改善肝纤维化。

Objective To assess the therapeutic effects of Huatan Xingyu （HTXY） decoction on experimental hepatic fi-brosis in rats. Methods Sixty adult Wistar rats were included into 6 groups （n=10 in each）：normal control group, model control group, Fufang Biejia Ruangan group, low- dose HTXY group, middle- dose HTXY group and high- dose HTXY group. The corre-sponding drugs were given for 6 weeks. After treatment, the hydroxyproline（Hyp） levels in hepatic tissues were detected by alkali hydrolysis method and the pathological changes of hepatic fibrosis were observed by HE staining and picric acid- sirius red staining. Results Compared to normal control and model control groups, the degree of hepatic fibrosis were significantly atten-uated in Fufang biejia ruangan group and HTXY decoction groups （P〈0.05）, and more markedly in middle- dose group. The Hyp levels in hepatic tissues were also significantly decreased in middle- dose HTXY group （P〈0.05）. Conclusion HTXY decoction can improve hepatic fibrosis and reduce hepatic tissue Hyp levels in rats with experimental hepatic fibrosis.