肝素对大鼠thy-1肾炎肾小球细胞增生和u-PA/PAI-1表达的影响

Effect of Heparin on the Cell Proliferation and u-PA/PAI-1 Expression on Glomeruli in Rat Anti-thy1 Glomerulonephritis

目的 研究u PA及其抑制物PAI 1在大鼠thy 1肾炎中的表达及意义 ,并探讨肝素治疗对其表达的影响。方法 应用ABC免疫酶标法及图像分析技术 ,观察对照组、肾炎组和肝素组动物模型肾小球u PA和PAI 1的表达情况 ,并与光镜下肾小球细胞计数作相关性分析。结果 肝素组动物在其治疗后 7、14、2 1d时 ,肾小球细胞数明显减少 ,与肾炎组比较P值分别小于 0 .0 5或 0 .0 1:肾炎组、肝素组肾小球u PA和PAI 1的表达均高于对照组 ;于实验 3、7、14和 2 1d时 ,肾炎组动物的肾小球细胞数增多与u PA和PAI 1的表达呈正相关 (P <0 .0 5或 0 .0 1) ;肝素组于实验 3、7d时 ,肾小球细胞数的减少与PAI 1表达降低有关 (P <0 .0 5 )。结论 大鼠thy 1肾炎的肾小球细胞数增多与其u PA和PAI 1表达程度呈正相关 ;肝素治疗可减轻大鼠thy 1肾炎肾小球细胞增生 ,其作用机制可能与病程早期对肾小球PAI

Purpose To investigate the significance of u PA and PAI?1 expression on the glomeruli,and the effect of heparin on their expressions in rat anti thy1 glomerulonephritis. Methods We analyzed the cell proliferation and the expression of u PA/PAI?1 on the glomeruli by immunohistochemistry and quantitative analysis of immunostaining. Results The cell proliferation of the glomeruli decreased significantly at 7 th,14 th,21 st day after heparin treatment in comparison to the glomerulonephritic group( P <0.05 or 0.01).The expression of u PA and PAI?1 on the glomeruli in glomerulonephritic and heparin treated groups was higher than that in the control group.At 3 rd,7 th,14 th,21 st day,the glomerular hypercellularity in the glomerulonephritic group was closely related to the increased expression of u PA and PAI?1( P <0.05 or 0.01).At 3 rd,7 th day,the decreased cell proliferation of the glomeruli in heparin treated group had close relationship with the decreased expression of PAI?1( P <0.05). Conclusions In rat anti thy 1 glomerulonephritis model,the expression of u PA and PAI?1 increased with glomerular hypercellularity;heparin treatment can decrease the extent of glomerular hypercellularity in rat anti thy 1 glomerulonephritis.The treatment function of heparin might be related with the inhibitory effect of PAI?1 expression on the glomeruli.