中华按蚊不同株转铁蛋白基因表达水平的比较研究（英文）

Comparative study of transferrin gene expression level from different strains of anopheles sinensis

定量RT-PCR分析表明,中华按蚊溴氰菊酯抗性品系的转铁蛋白转录水平比敏感品系高11.5倍,现场采集品系比敏感品系高8.3倍。不同发育阶段的基因表达差异分析表明,成年雌蚊转铁蛋白mRNA的表达水平最高,其次是三龄幼虫和卵。中华按蚊溴氰菊酯抗性品系和本地收集的菌株有3到4倍的增加相比,和敏感品系相比,中华按蚊溴氰菊酯抗性品系和现场采集品系的转铁蛋白表达水平均有3到4倍的增加。这些结果表明,转铁蛋白的表达水平与中华按蚊溴氰菊酯的抗性水平的提高有同步性,提示转铁蛋白对中华按蚊溴氰菊酯对杀虫剂的抗性形成具有一定的作用。

Quantitative RT-PCR analysis indicated that the Anopheles sinensis transferrin transcription level was 11.5-fold higher in deltamethrin resistant strain and 8.3 fold higher in local collected strain than that of sensi- tive strain. The maximal level of transferrin mRNA was detected in adult female mosquito,followed by the 3rd instar larvae and the egg. Anopheles sinensis deltamethrin resistant strain and local collected strain have 3-to 4-fold increase of transferrin transcription compared to sensitive strain. These results indicated the transferrin express level was enhanced synchronously with the deltamethrin resistance level of Anopheles sinensis,and transferrin may confer some deltamethrin resistance in Anopheles sinensis.