微生物比浊法测定硫酸庆大霉素颗粒效价

Determination of the potency of Gentamicin Sulfate Granules by turbidimetric method and analysis

目的 建立微生物比浊法测定硫酸庆大霉素颗粒效价的方法.方法 以金黄色葡萄球菌为试验菌,菌液浓度为1.0%,培养测定时间为3 ～ 4h.结果 硫酸庆大霉素浓度在0.2 ～ 1.0U/ml 的范围内,浓度的对数与吸光度成良好的线性关系：A ＝ 0.6214 - 0.7187lgC（r ＝ 0.9989）,平均回收率为99.2%,RSD 为0.25%.结论 微生物比浊法测定硫酸庆大霉素颗粒的效价简便、快速.

Objective Establish a turbidimetric method for determination the content of Gentamicin Sulfate Granules. Methods The test organism was staphylococcus aureus, adjusted spore suspension of staphylococcus aureus inoculating with a 1.0% ratio, cultured at 37℃ for about 3-4h. Results The calibration curve was liner in the range of 0.2-1.0U/mL ,while the calibration curve linear equation is A=0. 6214-0. 71871gC （r=0. 9989）, and the average recovery is 99.2 % with RSD of 0.25 %. Gentamicin sulfate in concentration in 0.2- 1.0U/mL, within the scope of the concentration of the logarithm and absorbency into good linear relationship between the linear equation ： A= 0. 6214-0. 7187lgC（ r = 0. 9989）, and the average recovery is 99.2 %, and RSD is 0.25 %. Conclusion Microbial turbidity method for the determination of Gentamicin Sulfate Granules titer is simple, accurate and fast.