冠心病冠脉侧支循环与血管紧张素转换酶基因多态性的相关研究

Relationship between coronary collateral circulation and angiotensin converting enzyme gene polymorphism of patients with coronary heart disease

以 6 8例冠心病患者为研究对象 ,从分子遗传学角度探讨冠脉侧支循环的建立机制。结果显示 :1冠心病组与对照组基因型构成比有显著性差异 (P<0 .0 5 )。冠心病组的 DD(Deletion,D、 Insertion,I)基因型频率与 D等位基因频率显著高于对照组。 2 4周内未服用血管紧张素转换酶 (ACE)抑制剂冠心病组与对照组的 DD与II,ID与 基因型之间 ACE水平有极显著性差异 (P<0 .0 1)。 3三支病变组较二支病变组及一支病变组的基因型构成比有显著性差异 (P<0 .0 5 )。 4冠脉侧支循环 0级组、 1级组与 2级组、 3级组的基因型构成比有显著性差异。结论 :1DD基因型或 D等位基因提示血浆高 ACE水平。 2随 D等位基因频率增高 ,病变冠脉支数增加。 3DD基因型或 D等位基因提示冠脉侧支循环不易建立。

WT5”BZ]The studies were performed in 68 adult patients with coronary heart disease (CAHD)and 69 healthy subjects who were blood donors Polymerase chain reaction technique and colorimetry method were used to detect the subjects’ ACE genotypes and plasma ACE levels respectively Results showed that a significant increase in the freqnency of DD genotype versus non-DD genotype was found among the patients with CAHD compared with heal thy control subjects (P<0 05),The frequency of DD genotype (0 54)and D alleles(0 68)was higher in the CAHD group than in normal subjects (0 33 and 0 51,respectively) There were strongly significant differences were observed between DD and II genotype,ID and II genotype(P<0 01) Triple-vessel disease was more strongly associated with D alleles than single-vessel disease and two-vessel disease There was significant diffrences between grade0、grade 1 and grade2、grade 3,respectively This suggest that a deletion polymorphism is associated with higher plasma ACE levels A deletion polymorphism increases risk for mutivessel disease A deletion polymorphism might indicate that coronary colleteral circulation didn’t develop satisfyingly [WT5”HZ]