1例HIV-1感染者HIV辅助受体CCR5基因的核酸序列分析

Sequence Analysis of CCR5 Gene in a HIV- 1 Infected Individual

目的 研究中国人HIV辅助受体CCR5基因变异的频率和类型,观察CCR5基因变异HIV感染和发病的关系。方法 采集6例河北籍HIV感染者和部分配偶、子女的共14份血液标本,分离外周血淋巴细胞,提取mRNA,用RT-PCR方法对CCR5基因进行扩增分析。对其中1例纯合的CCR5缺失缺陷型感染者的扩增片段进行了克隆和序列测定。结果 在所检测的14份样品中,发现1例纯合的CCR5基因缺失缺陷型。对该片段的序列测定表明,其缺失片段为60个碱基,与国外报道的32个碱基缺失有28个碱基重叠。结论 首次在中国人群中发现了纯合的辅助受体CCR5基因缺失缺陷型,对这种基因变异与HIV感染和发病关系的研究正在进行中。

Objective To investigate the frequency and difference of the HIV-1 co-receptor OCR5 and study the relationship helween the mutation of CCR5 and the AIDS progression. Methods The total mRNA was obtained from PBMC of the six HIV infected individuals and some of their spouses or their children. RT-PCR was employed to amplify the CCR5 gene. Then, the fragment loading the deletion was cloned to I vector and scquenced. Results One of the fourteen subjects was found to be a homozygote who has a deletion of CCR5 gene. Sequence result showed that the deletion was 60 nucleotides, of which 28 nucleotides were the same as the 32 nucleotides deletion published abroad. Conclusion This study is the first one which reports the hornozygous deletion of OCR5 gene in Chinese people and, the relationship between the CCR5 mutation and AIDS progression is being studied.