脑溢安对实验性脑出血大鼠细胞色素c氧化酶活性的影响

Effects of Nao-Yi-An granule on activity of cytochrome c oxidase in hippocampus of experimental intracerebral hemorrhagic rats

目的 :从神经元能量代谢的角度 ,探讨脑溢安治疗脑出血的作用机制。方法 :采用胶原酶诱导大鼠脑出血模型 ,以组织化学方法结合灰度值半定量法测定细胞色素c氧化酶 (cytochromecoxide,CO)活性 ,配合Nissl’s染色和细胞计数。结果 :脑溢安可维持大鼠海马CA1区CO活性 ,减少细胞缺失。结论 :脑溢安在脑出血中的神经元保护机制可能与其维持CO活性 ,改善细胞有氧代谢 。

Objective: To explore the neuronal protective effects of a traditional Chinese medicine complex Nao Yi An granule(NYA) on experimental intracerebral hemorrhage(ICH) in rats. Methods: Eighty collagenase induced ICH rats were used. At 12?h, 24?h, 2?d,4?d, 7?d, cytochrome c oxidase(CO) activity was measured with histochemistry combined with gray scale scanning. Pyramidal cell(PC) counting was also employed following Nissl's staining. Results: The CO activities in NYA treated group were less than those in model control group, and the neuronal loss was less in NYA treated group than that in model control group. Conclusions: The neuronal protective mechanism of NYA following ICH may be related with its maintaining activity of cytochrome c oxidase, which contributes to improving cellular aerobic metabolism and increasing energy supply for cells. [