摘要
目的 :探索抑癌基因P5 3基因的表达水平与卵巢肿瘤的临床和生物学行为之间是否存在一定的关系。方法 :应用免疫组化SP法检测P5 3在 71例卵巢上皮性肿瘤中的表达情况 ,并应用显微切割获取DNA和聚合酶链式反应 (PCR)—单链构象多态性分析 (SSCP)检测P5 3第 8外显子 (exon8)突变的情况。结果 :卵巢癌中P5 3蛋白阳性率为 5 1 6 % ,正常及交界性肿瘤中均为阴性 ,差异有极显著性意义 (P <0 0 0 1 )。P5 3蛋白阳性表达率在进展期 (Ⅲ/Ⅳ期 )较早期 (Ⅰ/Ⅱ期 )显著为高 ,差异有显著性意义 (P<0 0 5 )。P5 3表达水平与卵巢癌的组织学分级无关。结论 :P5 3蛋白表达异常在卵巢癌的进展过程中可能发挥有重要作用。卵巢癌中exon8可能不是P5
Objective:To search for the relationships among the expressive level of P53 gene,the clinical and biological behavior of epithelial ovarian tumor.Methods:In this study,the expression of P53 has been investigated by immunohistochemical study in a series of 71 patients,with benign (30 patients),borderline(10 patients) and malignant(31 patients) epithelial ovarian tumors.Furthermore,P53 exon8 mutant rate was analysed by single strand conformation polymorphism(SSCP).SSCP was performed on cases that has detectable P53 protein.The DNA templates were achieved through microdissection.Results:The levels of P53 protein were undetectable in patients with benign cystadenoma and borderline adenoma,detectable in a significant proportion (51.6%) of carcinoma (P<0.01) P53 expression was significantly more frequent in advanced ovarian carcinomas (stage Ⅰ/Ⅱ vs Ⅲ/Ⅳ,P<0.05).No P53 exon8 point mutation has been detected.No clear correlation could be observed between the expression levels of P53 and the histological grade of ovarian carcinoma.Conclusion:P53 expression may play a critical role in the progression of ovarian carcinoma. [
出处
《内蒙古医学杂志》
2000年第6期416-418,共3页
Inner Mongolia Medical Journal
