酶解水提法从胡萝卜中提取水溶性β-胡萝卜素

Extraction of Water-Soluble β-carotene from Carrots by Enzymatic Hydrolysis

研究了酶法水解从胡萝卜中提取水溶性β-胡萝卜素的关键工艺参数。单因素实验结果表明,较优提取工艺条件为:取鲜胡萝卜50g,直接打浆后加入100mL 0.2g/100mL的吐温-80溶液,再加入1.5g纤维素酶和0.4g果胶酶,经超声波破碎40min后,调pH6.0,于40℃搅拌2h后加入2g硅藻土抽滤。响应面分析优化得到的较优提取条件为:果胶酶0.49g,酶解pH 6.08,超声波时间41.58min,优化后水溶性β-胡萝卜素提取率为12.23μg/g。

The key technical parameters by enzymatic hydrolysis to extract water-soluble β-carotene from carrots were investigated.The optimal technological process determined by single factor experiment was as follow：50 g fresh carrot was homogenated,and then 100 mL 0.2 g/dL Tween-80,1.5 g cellulase and 0.4 g pectinase were added ;the carrot pulp was smashed for 40 min by ultrasonic wave,then pH was adjusted to 6.0;after stirring for 2 h at 40 ℃,2 g diatomite was added and finally filtrated.The optimal factors obtained from response surface analysis were 0.49 g pectinase,enzymatic hydrolysis pH was 6.08 and ultrasonic time was 41.58 min.Under above conditions,the extraction yield for water solubleβ-carotene reached 12.23 μg/g.