黄曲霉菌感染花生的不同检测方法的应用

Different Detections on Peanut Infected by Aspergillus flavus

通过黄曲霉菌对不同品种花生感染后的检测技术的应用,不仅比较方法的适用性,更能从研究品种中发现对黄曲霉菌抵抗能力强的品种。从辽宁省风沙所和葫芦岛市选用18种花生品种,用黄曲霉NRRL3357菌株接种花生仁培养7 d,根据传统分级标准得到的感染指数,分析花生的抗黄曲霉感染的能力;利用半定量薄层层析法和精确定量高效液相色谱法检测到的黄曲霉毒素质量浓度,确定花生的抗黄曲霉毒素合成的能力。同一品种表观评估法观测的黄曲霉感染指数与薄层色谱法和高效液相色谱法测定的黄曲霉毒素的质量浓度基本一致,鉴定出2个高抗黄曲霉感染和黄曲霉毒素质量浓度低的花生品种,分别是阜花11和白沙1016。

Different detection techniques were applied on detecting situations of peanut infected by Aspergillus flavus （A.flavus）,the applicability of different techniques can be compared with each other while the potential resistant peanut lines can also be screened out from the peanut lines.The 18 peanut lines,chosen from Institute of Windy and Sandy Soil Improvement and Utilization of Liaoning Province and Huludao City,the peanut lines were inoculated with A.flavus NRRL3357 and cultured for 7 days.The resistance capacity of peanut against A.flavus was assessed according to the level of infection index deduced from the traditional classification standard ;the capability of blocking the biosynthesis of aflatoxin was estimated on the basis of the value measured from TLC and HPLC.The levels of infection index of different peanut lines were almost accordant with the values of TLC and HPLC.Two resistant peanut genotypes,Baisha1016 and Fuhua 11,were identified high resistance against A.flavus and low content of biosynthesis of aflatoxin.