胎盘组织中促肾上腺皮质激素释放激素的免疫组化研究

Immunohistochemistry identification of corticotropin-releasing hormone positive cells from the human placenta

目的 :了解胎盘组织中促肾上腺皮质激素释放激素 (CRH)的表达量与分布 ,获取孕妇外周血中高浓度 CRH来源于胎盘的直接证据。 方法 :采用免疫组织化学 L SAB法显示胎盘组织中 CRH的表达量及其分布。 结果 :早期绒毛合体滋养层细胞 CRH表达量较低 ,呈弱阳性 ;中期引产的胎盘组织合体滋养层细胞 CRH表达量有所增加 ,呈弱阳性至阳性不等 ;晚期胎盘组织合体滋养层细胞 CRH表达量进一步增加 ,均呈强阳性。 结论 :妊娠晚期及分娩时 ,母体血中高浓度的

Objective: To identify corticotropin releasing hormone(CRH) positive cells from the human placenta. Methods: Using immunohistochemistry to show the cellular expression of placental CRH. Results: During human pregnancy, placental CRH level rose markedly from undetectable amounts prior to 20 weeks gestation to nearly the peak level. Conclusion: The presence of CRH in maternal plasma is attributed to the placental production and subsequent release of this hormone into the maternal circulation. [