IL-8在颌面部火器伤间接损伤牙髓组织中的表达

Expression of IL-8 in pulpal tissue injured by maxillofacial firearm trauma

目的 :研究颌面部火器伤间接损伤牙髓中白介素 8(IL - 8)的表达情况 ,初步探讨 IL - 8在此损伤炎症中的作用 ,以期为火器伤牙髓间接损伤的临床诊治提供理论依据。方法 :采用直径 5 .80 mm制式枪弹致伤狗下颌部的模型 ,并利用免疫组化染色及定量分析方法检测颌面部火器伤后不同时间、下颌不同牙齿的牙髓组织中 IL- 8的动态变化。结果 :IL- 8在伤后牙髓组织中有阳性表达 ,并随时间的改变 ,呈动态变化过程。IL- 8的阳性细胞主要包括浸润炎细胞、血管内皮细胞以及部分成纤维细胞等 ,它不仅与炎细胞的聚集和活化呈一致性 ,而且与牙髓组织损伤程度和时间呈正相关。 结论 :IL- 8在颌面部火器伤牙髓间接损伤的创伤炎症反应的发生、发展。

Objective: To provide theoretical basis and experimental evidence for diagnosis and therapy of dental pulp indirect injuries by gunshot wounds. Methods: The levels of IL 8 in different stages were studied using immunohistochemical method to evaluate its functions in inflammation process of dental pulps. Results: IL 8 expression was detected by immunohistochemical assay in postwound conditions. The results showed that positive staining of IL 8 was observed predominantly on infiltrating inflammatory cells, vascular endothelial cells and some fibroblasts, which was consistent with the recruitment and activation of inflammatory cells, and with the severity duration of pulpal tissue injury and response. Conclusion: These results indicate that IL 8 is involved in regulating physiologic responses of dental pulp injuries in maxillofacial trauma by a projectile. [