云南河谷构树的遗传多样性研究

Analysis on Genetic Diversity of Broussonetia papyrifera Distributed in River Basin of Yunnan Province

以云南省境内金沙江、元江、红河和怒江流域自然分布的构树为试材,采用AFLP分子标记技术对90份构树种质资源进行了遗传多样性分析研究。结果表明:筛选出的7对引物组合共扩增获得786条清晰可辩的条带,其中,多态性带632条,多态性条带百分率达80.4%,平均每对引物组合检测出90.3个多态位点。分布于4条水系流域的构树居群间,金沙江流域构树居群的遗传多样性水平最高,Nei’s基因多样性指数为0.145 5,而元江流域构树居群的遗传多样性水平最低,Nei’s基因多样性指数为0.112 9。4个构树居群间的遗传分化系数为0.038 6,表明构树的遗传变异主要存在于居群内不同个体之间。在遗传距离为0.003时,4个构树居群可分为2组,第1组由金沙江流域的构树居群构成,第2组包含分布于红河、怒江和元江流域的3个构树居群。

Ninty Broussonetia papyrifera samples were collected from four river basins in Yunnan province and used as materials, and AFLP markers were employed to analyze their genetic diversity. The results showed that total 786 unambiguous bands were revealed from selected 7 primer combinations, of which 632 bands were polymorphic, and the ratio of polymorphic bands was 80.4 %. An average of 90.3 polymorphic bands was detected from each primer combination on average. Among four B. papyrifera populations, the population of Jinsha - river Basin held the high- est level of genetic diversity, the Nei＇ s gene diversity being 0. 145 5, while the population of Yuanjiang-river Basin held the lowest level of genetic diversity, the Nei＇s gene diversity being 0.112 9. The genetic differentiation coeffi- cient was 0. 038 6, which indicated that the genetic variation of individuals within population being the main sources of genetic diversity in B. papyrifera. The four populations of B. papyrifera could be divided into two groups with the genetic distance threshold 0. 003. The group I consisted of only the population of Jinsha-river Basin, and group II in- cluded the other three populations of B. papyrifera. This research results could provide some references for the pro- tection and utilization of B. papyrifera resources.