瑞舒伐他汀预处理影响小鼠心肌缺血再灌注炎症因子和细胞凋亡

Expression effect of hs-CRP and TNF-α on rosuvastatin pretreatment of myocardial ischemia/reperfusion in mice

目的:探讨瑞舒伐他汀预处理对小鼠心肌缺血/再灌注后炎症因子的影响及其可能的心肌保护机制。方法:C57小鼠72只随机分为3组:假手术组(Sham组)、心肌缺血/再灌注组(MI/R组)、心肌缺血/再灌注+瑞舒伐他汀预处理组(MI/R+R组);建立小鼠MI/R模型;ELISA法检测血清高敏C反应蛋白(hs-CRP)和肿瘤坏死因子-α(TNF-α)的含量,Evansblue/TTC染色测定心肌梗死面积,TUNEL染色检测心肌细胞凋亡。结果:MI/R+R组的hs-CRP和TNF-α含量明显低于MI/R组(P<0.01)。MI/R+R组与MI/R组相比,心肌梗死面积明显减少(P<0.05),心肌细胞凋亡显著减轻(P<0.05)。结论:瑞舒伐他汀可能通过减少炎症因子合成或释放,进而抑制心肌细胞凋亡,减轻小鼠心肌缺血/再灌注损伤。

Objective： To study the effect of inflammatory factor and the eardiopro teetive mechanism on rosuvastatin pretreatment of my- oeardial ischemia/reperiusion in mice. Methods： Seventy-two male C57 mice were randomly divided into three groups： sham group（group Sham）, myocardial isehemia/reperfusion group（group Ml/R）and rosuvastatin pretreatment group（group MI/R＋R）. Mouse MI/R model was es- tablished. Concentrations of serum high sensitivity C-reactive protein （hs-CRP） and tumor necrosis factor alpha （TNF-a） were measured by ELISA. Myocardial tissue samples were stained with TTC to detect the size of myocardial infarction. Myocardial apoptosis were observed hy TUNEL stained. Results： Concentrations of sermn hs-CRP and TNF-c~ were significantly lower in group MI/R＋R than in group MI/R （P〈 0.01）. Myocardial infarctionsize was smaller（P〈0.05） and myocardial apoptosis was reduced（P〈0.05） in group MI/R＋R. Conclusion： Rosu- vastatin pretreatment protects myocardium against I/R injury and inhibites apoptosis possibly by reducing the synthesis or release of inflam- mation factors.