糖尿病大鼠神经功能和神经山梨醇的变化及黄腐酸钠的作用

Nerve function and nerve sorbitol content changes in diabetic rats and the effect of sodium fulvate

目的 观察实验性糖尿病大鼠的神经功能与神经山梨醇变化及中药黄腐酸钠对其影响。方法 腹腔注射链脲佐菌素建立糖尿病大鼠模型。给予黄腐酸钠防治 6个月后 ,测定大鼠的血糖、糖化血红蛋白、神经山梨醇含量 ,利用热痛测试仪及热板试验测定痛阈变化。结果 (1)黄腐酸钠治疗未显示降血糖作用。 (2 )糖尿病非治疗组热痛缩腿反应潜伏期 (6 .43± 0 .48)秒明显缩短 ,热板试验显示在非伤害性温度下 (4 0℃ )即出现痛觉反应 ,44℃时每分钟缩腿回避次数 (3 .42± 0 .5 9)次 /分 ,较正常组 (0 .88± 0 .36 )次 /分明显增多 (P均 <0 .0 5 ) ;神经山梨醇 (2 79.0 3± 6 3.37)nmol/mg显著增高 (P <0 .0 5 )。 (3)糖尿病治疗组热痛缩腿反应潜伏期 (7.5 6± 0 .46 )秒 ,较糖尿病非治疗组明显延长 ,热板试验 44℃下每分钟缩腿回避次数显著减少 ,从 (3 .42± 0 .5 9)次 /分降至 (2 .40± 0 .5 1)次 /分 ;神经山梨醇明显下降 ,(16 3 .45± 13 .2 4)nmol/mg ,低于未治疗组 ,接近非糖尿病对照组水平 (15 2 .6 0± 2 6 .5 0 )nmol/mg。 (4 )神经山梨醇与血糖、糖化血红蛋白呈显著正相关 ;热痛缩腿反应潜伏期与血糖、糖化血红蛋白、神经山梨醇呈显著负相关。结论 糖尿病时存在痛觉过敏和神经山梨醇异常 ;二者与高血糖状态?

Objective To observe the changes of nerve function and nerve sorbitol in diabetic rats and to explore the effect of sodium fulvate (SF). Methods Diabetic rats were induced by intraperitoneal injection of streptozotocin. One group of diabetic rats were given SF for six months. Plasma glucose, HbA 1c and nerve sorbitol were measured, and pain threshold was detected by thermalpain apparatus and hotplate test. Results (1)There was no effect of SF on hyperglycemia in diabetic rats. (2)The withdrawal latency was significantly decreased and nerve sorbitol content was significantly increased in untreated diabetic rats. (3)Untreated diabetic rats showed pain reaction at non noxious temperature (40℃) and the times of hind paw withdrawal reflex per minute at 44℃ were increased in hotplate test 〔(3.42±0.59)/min〕 as compared with non diabetic control group 〔(0.88±0.36)/min〕 (P<0.05), while in SF treated group the withdrawal latency was prolonged (7.56±0.46)/sec and the times of hind paw withdrawal reflex/min at 44℃ were decreased in hotplate test from (3.42±0.59)/min to (2.40±0.51)/minascomparedwithuntreateddiabetic rats (P<0.05). The level of nerve sorbitol was significantly reduced by SF treatment (P<0.05). (4)Nerve sorbitol level was positively correlated with blood glucose and HbA 1c levels. The withdrawal latency was negatively correlated with blood glucose, HbA 1c and nerve sorbitol levels. Conclusion Diabetic rats show hyperalgesia and high levels of nerve sorbitol content which are related to hyperglycemia and can be allevialed by SF treatment.