摘要
目的 :建立一个快速鉴别区分鬼臼、龙胆、威灵仙的分析方法。方法 :以鬼臼所含毒性成分为分析对象 ,选择适宜的HPLC条件 ,建立了指纹图谱分析方法 ,并对该方法进行了考察。结果 :方法学考察结果表明 ,本研究建立的分析方法有较好重现性与复现性 ;鬼臼指纹图谱与威灵仙、龙胆HPLC图谱截然不同。结论 :HPLC指纹图谱分析法可简便快速地鉴别区分鬼臼 (桃儿七 )、龙胆、威灵仙 ,并可用于不同来源鬼臼的鉴别。
Objective: To establish a method for indentification of the podophyllum emodi species, Radix Clematis and Radix Gentiana species. Methods: HPLC/UV fingerprint analysis method of toxic ingredient podophyllotoxin and its derivatives were developed, and the method had been evaluated. Results: The methodological evaluation showed that this method had a good repeatability and reproducibility, and different samples had different HPLC fingerprints. Conclusion: This method can be used to differentiate podophyllum emodi from two commonly used medicinal herbs of a different genus but having similar appearance, Radix Clematis and Radix Gentiana.
出处
《中成药》
CAS
CSCD
2000年第12期819-824,共6页
Chinese Traditional Patent Medicine
关键词
鬼臼
毒性成分
指纹图谱
威灵仙
龙胆
中药
鉴别
podophyllum
toxic ingredient
HPLC fingerprint
Radix Clematis
Radix Gentiana
