摘要
目的：对金丝桃不同部位提取物的清除活性氧活性进行了实验研究。方法：用化学发光法研究了金丝桃提取物对 Ｏ２、Ｈ２Ｏ２的清除作用；用分光光度法研究了提取物对·ＯＨ的清除作用以及对Ｈ２Ｏ２引起红细胞损伤和由Ｆｅ２＋－Ｖｉｔ Ｃ引起线粒体膨胀的保护作用。结果：金丝桃提取物能有效地清除Ｏ２、Ｈ２Ｏ２和·ＯＨ，对Ｈ２Ｏ２引起的红细胞损伤有一定的保护作用，同时能抑制由Ｆｅ２＋－Ｖｉｔ Ｃ引起的线粒体膨胀。结论：金丝桃提取物有良好的清除活性氧活性。
Objective: To study the effect of the extracts of Hypericum Chinense on eliminating active oxy-radi- cal. Methods: Chemiluminescence method and spectrometric method were used to measure the eliminating effect of Hypericum chinense on superoxide anion (O2 ) .hydrogen peroxide (H2O2 ) and hydroxyl radical (.OH ). Its protective effects on damage and lipid peroxidation of red blood cell induced by H2 O2 and mitochondria swelling in- duced by Fe2 + -Vit C were also investigated. Results: The extracts of hypericum chinense could eliminate O2 -. H2O2 and. OH effectively. They could also prevent the damage and lipid peroxidation of red blood cell induced by hydrogen peroxide and the mitochondria swelling induced by Fe2 + -Vit C. Conclusion:The results showed that Hypericum chinense was effective in eliminating active oxy-radical.
出处
《广东药学院学报》
CAS
2000年第4期282-284,共3页
Academic Journal of Guangdong College of Pharmacy
基金
国家自然科学基金资助项目(NO39990570)
关键词
金丝桃
化学发光
脂质过氧化
自由基
灌木植物
hypericum chinense
chemiluminescence
lipid peroxidation
antioxidaion effect
free radicals
