实时荧光RT-PCR在人感染H7N9禽流感病毒检测中的应用

Application of real-time fluorescence RT-PCR to detect avian influenza A( H7N9) virus

目的评估实时荧光逆转录聚合酶链反应(RT-PCR)检测H7N9禽流感病毒的应用效果,为人感染H7N9禽流感监测和防控提供可靠的实验室检测数据。方法采集各级医疗单位人感染H7N9禽流感监测及可疑病例标本137例,同时使用4种实时荧光RT-PCR试剂分别检测上呼吸道标本和下呼吸道标本中H7N9禽流感病毒核酸。结果 H7N9禽流感病毒核酸阳性率为3.65%(5/137);H7N9禽流感病毒核酸阳性标本中上呼吸道标本检出率为60%(3/5),下呼吸道标本检出率为100%(3/3)。4种试剂符合率为100%。结论实时荧光RT-PCR能有效检出H7N9禽流感病毒核酸,检测下呼吸道标本有助于提高阳性检出率。

Objective To assess the application effect of real-time fluorescence reverse transcription-polymerase chain reaction （RT-PCR） to detect avian influenza A（HTNg） virus, and to provide reliable data to laboratory detection for avian influenza A （H7Ng） virus surveillance, prevention and control. Methods A total of 137 being monitored and suspected specimens from hospitals were collected, and the nucleic acids of avian influenza A（HTNg） virus in specimens from upper and lower respiratory tracts were detected by 4 kinds of real-time fluorescence RT-PCR kits. Results The positive rate of avian influenza A （ H7N9 ） virus was 3.65 % （ 5/137 ）. Among the positive specimens, the positive rate of upper respiratory tract was 60% （3/5）, and the positive rate of lower respiratory tract was 100% （3/3）. The coincidence rate of 4 kinds of kits was 100%. Conclusions Real-time fluorescence RT-PCR can detect the nucleic acid of avian influenza A （ HTN9 ） virus effectively. Specimens from lower respiratory tract contribute to improve the positive detection rate of avian influenza A （ H7N9 ） virus.