血管活性肠肽对缺血再灌注损伤大鼠脑内GFAP的影响

Effects of vasoactive intestinal peptide to the activation of GFAP after focal cerebral ischemia-reperfusion in rats

目的通过活体实验研究血管活性肠肽(VIP)对脑缺血再灌注后胶质纤维酸性蛋白(GFAP)动态变化的影响及星型胶质细胞活化增殖的情况,探讨VIP的神经保护作用。方法大鼠注射VIP后,用线栓法制作大鼠大脑中动脉再灌注(MCAO)模型。通过神经学评价对MCAO大鼠进行判定和筛选;应用免疫组化技术检测脑缺血区GFAP表达。结果 VIP注射后与对照组相比GFAP表达显著增加(P<0.05),在相同缺血区的星形胶质细胞反应上调增强。结论 VIP脑内注射可明显增强GFAP的表达,降低再灌注损伤程度;提示VIP可能具有体外神经保护作用。

Objective To investigate the effects of vasoactive intestinal peptide（VIP） on the dynamic changes of glial fibrillary acidic protein（GFAP） after focal cerebral ischemia-reperfusion in vivo and the proliferation of astrocyte, and discuss the neuroprotective effects of VIP. Methods In rats, VIP was given via injection, and middle cerebral artery occlusion （MCAO） model was set up by suture method. The evaluation and screening of rats with successful occlusion of MCAO were performed by neurological testing. The expression of GFAP was detected by immunohistochemistry. Results Compared with the control groups after VIP injection, GFAP expression significantly increased（P 〈 0.05）. The existing astrocyte response in the same ischemic area was upregulated. Conclusions VIP injection enhances the expression of GFAP and reduces the degree of cerebral ischemia-reperfusion injury. This study provides the evidence for the neuroprotective effects of VIP in vivo.