可舒胶囊对酒精性肝损伤小鼠肝组织Caspase-3与Caspase-8活性的影响

Effects of Ke Shu Capsule on Caspase-3 and Caspase-8 activity of hepatic tissue in mice with alcoholic liver injury

目的探讨可舒胶囊对酒精所致大鼠急性肝损伤的保护作用及机制。方法采用酒精灌胃辅以高脂饲料法复制急性酒精性肝损伤小鼠模型,以血清谷酰转肽酶(GGT)、血清丙氨酸转氨酶(ALT)和天门冬氨酸转氨酶(AST)水平为模型参考指标。50只雄性昆明小鼠随机分为5组:正常对照组、肝损伤模型组、可舒胶囊低、中、高剂量(0.78、1.56、3.12 g/kg)干预组,每组10只。检测小鼠肝组织天冬氨酸特异酶切的半胱氨酸蛋白酶(Caspase)-3,-8活性。结果模型组小鼠血清GGT[(4.52±0.26)U/L]、ALT[(173.09±8.79)U/L]、AST[(141.90±7.82)U/L]水平较正常对照组显著升高(P<0.01);可舒胶囊高、中、低剂量干预组小鼠血清GGT水平分别为(1.42±0.10)、(2.65±0.41)、(3.25±0.56)U/L;ALT分别为(72.19±8.19)、(78.52±9.72)、(98.95±10.70)U/L;AST水平分别为(70.27±8.19)、(77.46±5.94)、(94.57±5.26)U/L;较模型对照组显著降低(P<0.01)。模型组小鼠肝组织Caspase-3活性为(6.54±0.43)U/mgpro,Caspase-8活性为(2.71±0.23)U/mgpro,较正常对照组显著升高(P<0.01);可舒胶囊高、中、低剂量组小鼠肝组织Caspase-3活性分别为(2.57±0.33)、(3.20±0.30)、(4.32±0.35)U/mgpro;Caspase-8活性分别为(1.13±0.25)、(1.43±0.29)、(1.98±0.31)U/mgpro,较模型组显著降低(P<0.01)。结论酒精肝损伤小鼠肝组织Caspase-3与Caspase-8活性显著升高,可舒胶囊对其具有明显抑制作用。

Objective To investigate the protective effect of Ke Shu Capsule for acute liver injury in mice induced by alcoholic and its possible mechanism. Methods The liver injury models of mice were induced by infusing alcohol into stomach, and feeding with fat-rich diet at the same time, then GGT, ALT, AST levels were taken as model reference indicators. 50 male KM mice were randomly divided into 5 groups： control group, acute liver injury model group, and low, middle, high doses of Ke Shu Capsule groups （0.78, 1.56, 3.12 g/kg）. The Caspase-3 and Caspase-8 activities were assayed and compared between groups. Results Compared with control group, the serum concentrations of GGT [（4.52±0.26） U/L], ALT [（173.09±8.79） U/L] and AST [（141.90±7.82） U/L], hepatic tissue Caspase-3 activity [（6.54± 0.43） U/mgpro] and Caspase-8 activity [（2.71±0.23） U/mgpro], significantly increased in acute liver injury model group （P 〈 0.01）. Compared with acute liver injury model group, in high, middle, low doses of Ke Shu Capsule groups, the serum coneentrations of GGT [（1.42±0.10）, （2.65±0.41）, （3.25±0.56） U/L], ALT [（72.19±8.19）, （78.52±9.72）, （98.95± 10.70） U/L] and AST [（70.27±8.19）, （77.46±5.94）, （94.57±5.26） U/L], hepatic tissue Caspase-3 activity [（2.57±0.33）, （3.20±0.30）, （4.32±0.35） U/mgpro] and Caspase-8 aetivity [（1.13±0.25）, （1.43±0.29）, （1.98±0.31） U/mgpro] significant- ly decreased （P 〈 0.01）. Conclusion Ke Shu Capsule prevents liver from alcoholic impairment may be through de- creasing Caspase-3 and Caspase-8 aetivities.