鸭垂体中GH基因表达的发育性变化分析

Analysis on GH gene expression level in pituitary of embryonic and neonatal duck

以生长速度差异较大的‘高邮鸭’和‘金定鸭’为试验材料,采用实时荧光定量PCR方法检测2个品种鸭胚胎期和出雏早期垂体中GH mRNA的差异表达水平,探究生长激素(GH)基因在不同品种鸭胚胎期和出雏早期垂体中表达水平的发育性变化.从13胚龄到7日龄,垂体中均可检测到GH mRNA的表达,其发育性变化在品种间表现出不同的变化规律.‘高邮鸭’随胚(日)龄的增加,垂体GH mRNA水平一直大幅增加,胚龄间差异显著或极显著(P<0.05或P<0.01);‘金定鸭’在17胚龄和21胚龄间表达量差异不显著(P>0.05),在其他各胚龄间差异极显著(P<0.01).在胚胎期,在13、15、17、25胚龄,‘金定鸭’垂体中GH的表达量均高于‘高邮鸭’垂体中的表达量,但差异不显著(P>0.05),在21胚龄和出雏后第7d,‘金定鸭’垂体中GH的表达量显著低于‘高邮鸭’垂体中的表达量(P<0.05).研究表明,垂体中GH基因表达具有显著的胚(日)龄依赖性和明显的品种特征,垂体中GH基因的表达对动物早期生长具有重要影响.

This experiment was conducted to study the developmental change patterns of pituitary GH gene expression of different duck breed during embryonic and neonatal development. In present study, the materials of test were ＇Gaoyou＇ duck and ＇Jinding＇ duck,which had the great difference in growth rate. The GH gene expression patterns of pituitary were compared between ＇Gaoyou＇ and ＇Jinding＇ duck using real-time quantitative PCR method. The results showed that the GH mRNA was detected in two cluck breeds from E13（13 embryo age） to D7（7 days） in the pituitary. The GH mRNA in pituitary of ＇Gaoyou＇ was increased rapidly from E13 to D7 （P〈0. 05 or P〈0. 01）. While a different pattern exhibited in ＇Jinding＇ ducks,the GH mRNA in pituitary was increased rapidly from E13 to D7 （P〈0. 01）,except from E17 to E21 （P〉0.05）. On E13, El5, E17 and E25, the GH mRNA expression level in pituitary of ＇Jinding＇ were higher than that of ＇Gaoyou＇, but there were no significant difference （P〉0. 05）. But a reversed cor relation was observed on E21 and D7, the ＇ Gaoyou ＇ showed higher than the ＇ Jinding＇ （P〈0. 05）, displa ying a negative relationship with the hatching growth rate. The results indicated that the GH gene expres sion in pituitary has the embryo/days age-dependence and significant species characteristics,and has an im portant effect on the early growth of the animal.