狼尾草遗传多样性SRAP分析

SRAP analysis on genetic diversity of Pennisetum

利用SRAP分子标记技术分析了19份狼尾草资源间的遗传关系.结果表明:100对SRAP引物中,38对引物多态性较好,每对引物产生9～22条条带,共扩增出682条谱带,其中多态性条带为157条,平均每对引物组合产生4.13个多态性条带,表明在这19份狼尾草资源中具有较高的遗传多样性.经NTSYS pc2.01软件聚类分析,狼尾草品种间简单匹配相似系数为0.53～0.82.在相似系数为0.60时,可将19个狼尾草品种分为A、B、C 3类;在相似系数为0.67时,B类分为2个亚类,C类分为3个亚类.此结果与狼尾草形态学特征相配合,为鉴别狼尾草资源亲缘关系提供了科学依据.

The genetic relationship of 19 cultivars of Pennisetum were analyzed using SRAP molecule maker. The results showed that the 682 reproducible fragments of SRAP were produced by 38 of 100 pairs of primers the bands for each pairs of primers to all samples varied from 9 to 22,and 157 fragments （4. 13 per pairs of primers） were polymorphism which indicated that there was high genetic diversity among these 19 genotypes. From the results of NTSYS pc 2.01 software cluster analysis, the simple matching coefficient （SM） of the Penniseturn cultivars ranged from 0. 53 to 0. 82. When the similarity coefficient was 0. 60,the cultivars could be grouped into 3 categories, type A, B and C, based on cluster analysis of the SRAP finger- printing data. When the similarity coefficient was 0.67, type B had two subclasses~ type C had three subclasses. Combined with their morphological characteristics, the results could be applied for the objective basis to identify the genetic relationship of Penniseturn resources.