中国部分地区庚型肝炎病毒的基因型分析

Analysis of hepatitis G virus genotypes in some areas of China

目的 研究从中国部分地区不同人群分离的庚型肝炎病毒 (HGV)基因型分布。方法 从中国部分地区不同人群分离出 12株HGV ,对该 12株HGV 5′非编码区 (UTR)基因序列进行了测定 ,并与以前报告的 2 6株HGV(17株来自中国 ,9株来自其他国家 )做比较。结果 从同一例病人分离的 1株HGV的 5个克隆 ,其 5′ UTR序列的同源性为 99 1%～ 10 0 % ,说明HGV在同一病人体内变异很小 ,因此 ,根据 1个阳性克隆的序列可对HGV分离株进行基因分型。该 12株HGV 5′ UTR核苷酸序列的同源性为 96 8%～ 99 4% ,与 1型 (U36 380 )、2型 (U44 40 2 )和 3型 (D90 6 0 1)比较 ,其同源性分别为 88 0 %～ 89 7%、87 2 %～ 90 3%和 94 7%～ 96 3 %。表明该 12株HGV均属基因 3型。对该 12株HGV及以前国内外报告的 2 6株HGV种系发生分析表明 ,中国HGV分离株可能还分不同亚组。结论绝大多数中国株HGV属基因 3型 。

Objective To study the genotype distribution of hepatitis G virus (HGV) isolated from different population in some areas of China. Methods A total of 12 strains of HGV were isolated from different areas of China (three from Guangxi Zhuang Autonomous Region, three from Hebei Province, one from Heilongjiang, Jiangxi, Guangdong, Jiangsu, Anhui, Jilin Provinces and Xinjiang Autonomous Region each). The HGV 5′ untranslated region (UTR) of the 12 isolates was sequenced and compared with other 26 isolates previously reported (17 from China and nine from other countries). Results The nucleotide sequences of five HGV clones isolated from the same patient with HGV infection were identical, with the homology of 99 1%～100%, indicating that variation of HGV in the same human body was very limited. Genotyping could be based on the sequence of one positive clone isolated from a patient with HGV infection. The homology of the nucleotide sequences in the 12 isolates was 96 8%～99 4%, suggesting that they belonged to the same genotype. The homology of the 12 isolates was 88 0%～89 7%, 87 2%～90 3% and 94 7%～96 3%, respectively, as compared with HGV genotypes 1 (U36380), 2 (U44402) and 3 (D90601), which indicated that all the 12 HGV isolates belonged to genotype 3. Phylogenetic distance analysis of the 38 isolates suggested that HGV strains isolated from China could be further divided into different subgroups. Conclusion The majority of Chinese HGV isolates belonged to genotype 3 and they could be further divided into different subgroups.