人类白细胞抗原DRB1等位基因与幽门螺杆菌感染的关系

Study on the relations between HLA DRB1 alleles and Helicobacter pylori infection

目的 研究人类白细胞抗原 (HLA)DRB1等位基因与幽门螺杆菌 (Hp)感染的关系。 方法 用BioseedHp IgG定量酶联免疫试剂盒检测 46例胃癌、75例食道癌和 10 0例人群对照的Hp IgG抗体 ,用Biotest低解析水平HLA DRB酶联免疫探针杂交测定试剂盒检测HLA DRB1等位基因。结果 (1)Hp IgG阳性组DRB1 0 8基因频度显著高于Hp IgG阴性组 (13.1%vs 4.4% ;χ2 =11.14,P <0 .0 0 1)。Hp IgG阳性组DRB1 12基因频度显著低于Hp IgG阴性组 (5 .4%vs 11.3 % ;χ2 =4.49,P <0 .0 5 )。 (2 )胃癌组中DRB1 0 2基因频度显著高于对照组 ,而DRB1 0 7基因频度显著低于对照组 ,但在胃癌、对照的Hp IgG阳性组和Hp IgG阴性组之间的DRB1 0 2、DRB1 0 7基因频度差异均无显著性。结论 (1)HLA DRB1 0 8基因阳性可能增加对Hp的易感性 ,DRB1 12基因可能是抵御Hp感染的保护性基因。 (2 )DRB1 0 2基因阳性可能是胃癌的宿主遗传危险因素、DRB1 0 7基因可能是胃癌的保护性因素 ,但DRB1 0 2、DRB1 0

Objective In order to study the relation between human leukocyte antigen (HLA) DRB1 alleles and Helicobacter pylori (Hp) infection. Methods Hp IgG antibody from 46 gastric cancer (GC), 75 esophageal cancer and 100 population based controls were identified by Hp IgG quantitative enzyme immunoassay. Biotest HLA DRB enzyme linked probe hybridization assay kit (low resolution) was used to identify DRB1 alleles. Results (1) Frequency of DRB1*08 was significantly higher in Hp IgG positive group than in Hp IgG negative group ( 13.1 % vs 4.4 %, χ 2= 11.14 , P < 0.001 ). Frequency of DRB1*12 was significantly lower in Hp IgG positive group than in Hp IgG negatives ( 5.4 % vs 11.3 %,χ 2= 4.49 , P < 0.05 ).(2) Frequency of DRB1*02 in GC was significantly higher than that of controls. Frequency of DRB1*07 in GC was significantly lower than that of controls. However, neither the frequency of DRB1*02 between Hp IgG positive and Hp IgG negative groups nor the frequency of DRB1*07 between Hp IgG positive and Hp IgG negative groups showed significant differences in GC and controls. Conclusions (1) HLA DRB1*08 might serve a genetic risk factor for Hp infection while DRB1*12 might play a role of protecting effect against Hp infection. (2) DRB1*02 might be a genetic risk factor for GC while DRB1*07 might play a role of protecting effect against GC. However, the relations between DRB1*02, DRB1*07 and GC were not associated with Hp infection.