紫花苜蓿多元杂交后代的遗传多样性

Analysis on genetic diversity among multiple hybrid progenies of alfalfa

研究了苜蓿品种甘农3号、甘农5号及游客杂交后代与亲本的亲缘关系,选用ISSR分子标记进行遗传多样性分析,筛选出4条引物检测供试材料的遗传多样性。结果表明:4条引物共扩增出122个位点,均为多态性位点,共扩增出22条条带,其中,21条为多态行条带,多态性比率为95.45%,供试材料遗传多样性丰富。通过计算后代株系分别与3个亲本的遗传距离,速生16#与3亲本的遗传距离最小,为0.327;与3个亲本的平均遗传距离最大的是大叶1#,遗传距离为0.752。聚类分析结果表明:39份材料被聚为5类,A类包括速生1#、速生6#等14个株系;B类包括速生2#、速生3#等5个株系;C类包括速生4#、速生11#等13个株系;D类包括速生17#、速生26#等4个株系;速生18#单独被聚为一类。

In order to explore the genetic relationship between hybrid progenies of alfalfa cultivated species, involving Gannong 3# ,Gannong 5# ,Tourists and their parents, the genetic diversity was analyzed the by ISSR marker and 4 primers were screened to detect the genetic diversity of tested materials. The results showed that 4 primers with high polymorphism were screened and 122 sites were generated,which were all polymorphic sites. 22 informative ISSR markers were generated and showed a polymorphic band ratio of 95.45#. The genetic di- versity of tested materials was rich. Genetic distances between hybrid progenies and three parents were ana- lyzed,genetic distance between fast-growing 16# and the three parents was the smallest （0. 327）,while it be- tween big leaf 1# and the three parents was the biggest （0. 752）. Clustering results showed that 39 materials were clustered into 5 categories,there were 14 strains in Categories A,including 14 fast-growing 1# , fast-grow- ing 6# and so on;5 strains in Categories B,including fast-growing 2#- ,fast-growing 3# and so on; 13 strains in Categories C, including fast-growing 4# , fast-growing 11# and so on; 4 strains in Categories D, including fast- growing 17#,fast-growing 26# and so on;fast-growing 18# was clustered in one category.