摘要
通过形态学和16S rDNA序列分析方法,从绵羊瘤胃内容物中分离得到的细菌中鉴定出枯草芽胞杆菌。根据GenBank中已知Bacillus subtilis木聚糖酶基因序列设计带有酶切位点的引物,克隆到2条不同的木聚糖基因序列。通过酶切和连接等相关分子方法成功构建出以pMG36e为载体的表达木聚糖酶的重组质粒,转化到乳酸球菌粒MG1363后,均能高效表达,酶活性分别为28.29 U/mL和7.11 U/mL,比原枯草芽胞杆菌酶活2.17 U/mL高出13.04倍和3.28倍。
A cellulose degrade bacterium from the rumen of the sheep was indentified as Bacillus subtilis by using analysis of morphology and the conserved sequence 16S rDNA. Two different xylanase genes were cloned from the bacterium chromosome DNA by original strain of Bacillus subtilis. The two xylanase genes were connected with vector pMG36e to construct xylanase gene expression vector, and then transformed into E. coli and Lactococcus lactis MGr1363. Both them could express xylanase, and their enzyme activities reached 28.29 U/mL and 7.11 U/mL respectively,which were higher 13.04 times and 3.28 times than that of original strain of Bacillus sub tilis （ 2.17 U/mL）.
出处
《畜牧与饲料科学》
2013年第9期5-9,13,共6页
Animal Husbandry and Feed Science
