抗中性粒细胞胞浆抗体对溃疡性结肠炎诊断价值的研究

Clinical Evaluation of ANCA in Sera of the Patients With UC

目的 建立固定中性粒细胞－ＥＬＩＳＡ，分析血清中抗中性粒细胞胞浆抗体（ＡＮＣＡ）对溃疡性结肠炎（ＵＣ）的诊断价 值。方法 采用固定中性粒细胞－ＥＬＩＳＡ法检测 ２５份 ＵＣ血清、２６份细菌性结肠炎血清、１５份混合类型腹泻血清和 ５０份正 常对照血清中的 ＡＮＣＡ，对各组血清的吸光度（Ａ）值进行统计分析。结果 ＵＣ组 ＡＮＣＡ与其他对照组 ＡＮＣＡ之间的差异非 常显著（Ｐ＜０．００５）。ＵＣ组Ａ值超过正常对照组Ａ值２s以上病例达８０％，诊断特异性达８９％。结论 ＵＣ血清中存在有诊断 价值的 ＡＮＣＡ，固定中性粒细胞一ＥＬＩＳＡ可作为血清 ＡＮＣＡ检测的重要手段。

Objective The clinical evaluation of anti-Nutrophil cytoplasmic antibodies (ANCA) in sera of the patients with Ulcerative Colitis (UC). Methods The ANCA in sera from 25 patients with UC, 26 with Bacterial COlitis, 15 with Miscella- neons diarrheal illness and 50 normal subjects were detected with Fixed NeutroPhil Enzyme linked Immunosorbent Assay. Results The ANCA levels in sera of UC were obviously higher than those in other controls (P<0.005), 80% Of sera ANCA from UC had absorbance (A) beyond the values of normal subjects being plused 2s, the diagnostic specificity was 89%. Conclusion The results showed prescence of ANCA in sera with UC and this assay may be a valuble test for clinical diagnosis of UC.