肉牛关节大肠杆菌的分离鉴定及药物敏感性分析

Isolation, identification and drug sensitive analysis of E. coli in beef cattle joint

为了研究引起肉牛关节炎病原菌的种类及对抗生素的敏感性,采用细菌分离鉴定法、纸片扩散法对菌株进行鉴定和药物敏感性试验。结果表明:从2例病牛关节液中分离鉴定出2株大肠杆菌;用18种抗生素进行药物敏感性试验,分离菌株对环丙沙星、丁胺卡那霉素、氟哌酸、庆大霉素、先锋必素5种药物敏感,对复达欣、卡那霉素、头孢呋肟3种药物中介,对羧苄青霉素、先锋霉素V、强力霉素、美满霉素、复方新诺明、红霉素、氯霉素、四环素、先锋霉素IV、氧哌嗪青霉素10种药物产生耐药性。

Bacteria were identified and tested of drug sensitivities by using the bacterial isolation, identification method and disk diffusion method in order to study the types of pathogens which caused the beef cattle arthritis and antibiotic sensitivity. The results showed that 2 strains of E. coli were identified and separated from two cases of infected cattle synovial fluid. Results of 18 kinds of antibiotic susceptibility tests were as follows： E. coli was sensitive to ciprofloxacine, amikacin, norfloxacin, gentalnicin, cefoperazone intermediary sensitive to fortum, kanamycin cefuroxime had drug resistance to carbenicillin, cephalosporin V, doxycycline, minocycline, cotrimoxazole, erythromycin, chloramphenicol, tetracycline, neomycin Pioneer IV piperacillin penicillin.