合浦珠母贝不同壳基质蛋白基因的表达水平比较

Comparison of relative expression levels of shell matrix protein genes in the pearl oyster Pinctada fucata

采用荧光定量PCR技术分析了不同壳基质蛋白基因之间的表达水平差异、组织表达特征以及育珠对表达水平的影响。结果显示,msi31、aspein、msi7基因只在外套膜组织中表达;nacrein和n19基因除在外套膜表达外,在珍珠囊中也有表达;而accbp在外套膜、闭壳肌、性腺、消化腺、腮和珍珠囊中都有表达,在外套膜中表达量最高,其次为性腺。accbp、msi31、msi7和nacrein基因在育珠贝和非育珠贝外套膜中的表达水平有显著性差异,aspein与n19无显著性差异;其中msi31基因在育珠贝外套膜的表达量上调,accbp、msi7与nacrein下调。不论在育珠贝还是非育珠贝的外套膜组织中,msi31的表达水平最高,n19最低;但在珍珠囊中n19的表达量最高,且远高于在外套膜中的表达水平。

Real-time Quantitative PCR assay was performed to investigate the differences in relative expression levels （RELs） of shell matrix protein genes, tissue expression profiles and the expression differences in pearl-culturing oysters （PC） and non-pearl- culturing oysters （NPC）. The results showed that msi31, capein and msi7 only expressed in the mantle tissues; nacrein and hi9 expressed in both the mantle and the pearl sac; whereas accbp expressed in all the tissues, including mantle, adductor muscle, gonad, digestive gland, gill and pearl sac, with the highest expression level in the mantle, followed by the gonad. The RELs of accbp, msi31, msi7 and nacrein were significantly different between the mantle of pearl-culturing oysters （PC-M） and the mantle of non-pearl-culturing oysters （NPC-M）; aspein and n19 were not. Among others, msi31 upregulated while accbp, msi7 and nacrein downregulated. The REL of msi31 was the highest and n19 was the lowest in both PC-M and NPC-M. But in pearl sac, the REL of n19 was the highest and much higher than that in the mantle.