1株来自瘤胃液菌株RB1在降解秸秆中的作用

Biodegradation of Straw by Strain RB1 from Rumen Fluid

木质纤维素中木糖残基大量乙酰化,导致半纤维素的降解受阻。半纤维素支链水解断裂,可以解除其空间位阻效应,利于木质纤维素彻底降解。采用产乙酰酯酶活力较高的菌株RB1降解玉米秸秆和水稻秸秆。研究结果表明,菌株RB1对玉米秸秆和水稻秸秆中半纤维素降解率分别高达53.87%和51.67%。同时该菌株对秸秆中木质素降解率分别达到35.50%和35.01%。该菌株与其他降解纤维素能力较强的菌株共同发酵,会对木质纤维素类物质有更高降解率。该菌株在生物转化木质纤维素类物质方面,具有一定的潜在应用价值。

Large amount acetylation of xylose residues in lignocellulose conduce degradtion of hemicellulose being hindered. The hydrolytic breaking of lignocellulose branch could relieve its location hindering effect, and conducive to full degradation of lignocellulose. In the present study, a rumen bacterial RBI strain producing high-active acetyl-esterase was adopted to degrade maize stalk and rice straw. The results showed that degradation rate of hemicellulose of maize and rice stalks were as high as 53.87% and 51.67% respectively. At the same time the lignin degradation rate of the stalks and straw was as high as 35.50% and 35.01% respectively. When the strain was fermented together with high cellulose degradable strain, they could have even higher degradation rate of lignocellulose substances. The strain has a certain potential application value in the aspect of bioconversion of lignocellulose substances.