门静脉转流下阻塞性黄疸肝脏对缺血耐受性的研究

Ischemic tolerance of obstructive jaundice liver in rat with portal blood bypass

目的 探索在门静脉转流下阻塞性黄疸大鼠耐受入肝血流阻断的安全时限。方法 以胆管结扎后 1周大鼠门静脉转流下阻断入肝血流不同时程后 ,10d动物存活率、肝脏病理组织学改变及肝脏能量代谢功能损害的严重程度及可逆性。结果 胆道梗阻 1周大鼠阻断入肝血流 6 0min以内 ,术后 10d动物全部存活 ,其肝脏缺血再灌流损害可逆 ;阻断入肝血流时间超过 6 0min ,术后 10d动物存活率明显下降 ;入肝血流阻断 90min ,肝脏缺血再灌流损害严重而陷入失代偿状态。结论 胆道梗阻后 1周大鼠门静脉转流及常温下耐受入肝血流阻断的安全时限为 6

Objective To study the maximum tolerant limit of obstructive jaundice rats to hepatic inflow occlusion with portal blood bypass. Methods One week after biliary obstruction, the hepatic portal inflow vessels of rat were occluded with blood bypass in different durations, and then the livers were reperfused later. The survival rate of rats, the histopathological changes and energy metabolism of the liver were studied to obtain the safe tolerant limit of the animals to hepatic inflow occlusion in ordinary temperature under the circumstances of portal blood bypass. Results All rats with hepatic inflow occlusion within 60 min survived on day 10 after the occlusion, and the ischemia reperfusion injury of their livers was reversible and compensatory. However, the survival rates of rats with more than 60 min hepatic inflow occlusion decreased significantly. The liver injury of the rats with 90 min of hepatic inflow occlusion was severe and irreversible. Conslusion The safe tolerant limit of obstructive jaundice rats to normothermic hepatic vascular occlusion with portal blood bypass is 60 min.